The hydrolysis of N-benzoyl-L-argininamide by crystalline trypsin.

where e = the total enzyme concentration, s = the substrate concentration, t = the time, and K’ = the proteolytic coefficient (3) of the system. Reinvestigation of the above reaction over a greater range of initial substrate concentrations has now shown that the initial reaction velocity at 25’ is essentially independent of the initial substrate concentration for concentrations varying from 0.01 to 0.05 M and that, for these and lesser substrate concentrations, the initial reaction rates obey the MichaelisMenten (4) equation